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Genomic linkage map of the human blood fluke Schistosoma mansoni

Charles D Criscione, Claudia LL Valentim, Hirohisa Hirai , Philip T LoVerde and Timothy JC Anderson

We genotyped grandparents, parents and 88 progeny to construct a 5.6 cM linkage map containing 243 microsatellites positioned on 203 of the largest scaffolds in the genome sequence. The map allows 70% of the estimated 300 Mb genome to be ordered on chromosomes, and highlights where scaffolds have been incorrectly assembled. The markers fall into eight main linkage groups, consistent with seven pairs of autosomes and one pair of sex chromosomes, and we were able to anchor linkage groups to chromosomes using fluorescent in situ hybridization. The genome measures 1,228.6 cM. Marker segregation reveals higher female recombination, confirms ZW inheritance patterns, and identifies recombination hotspots and regions of segregation distortion. 

http://genomebiology.com/2009/10/6/R71

Genome Biology 2009, 10:R71

JUL/18/2009

Copyright(C) 2009 PRI (). All rights reserved.