Two types of alpha satellite DNA in distinct chromosomal locations in Azara's owl monkey
Prakhongcheep O, Hirai Y, Hara T, Srikulnath K, Hirai H, Koga A*
satellite DNA is a repetitive sequence known to be a major DNA component
of centromeres in primates (order Primates). New World monkeys form one
major taxon (parvorder Platyrrhini) of primates, and their alpha satellite
DNA is known to comprise repeat units of around 340 bp. In one species (Azara's
owl monkey Aotus azarae) of this taxon, we identified two types of
alpha satellite DNA consisting of 185- and 344-bp repeat units, which we
designated as OwlAlp1 and OwlAlp2, respectively. OwlAlp2 exhibits
similarity throughout its entire sequence to the alpha satellite DNA of
other New World monkeys. The chromosomal locations of the two types of
sequence are markedly distinct: OwlAlp1 was observed at the centromeric
constrictions, while OwlAlp2 was found in the pericentric regions. From
these results, we inferred that OwlAlp1 was derived from OwlAlp2 and
rapidly replaced OwlAlp2 as the principal alpha satellite DNA on a short
time scale at the speciation level. A less likely alternative explanation
is also discussed.
DNA Research 20 (2013): in press
Southern hybridization showing
green, OwlAlp1; red, OelAlp2
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