JAPANESE TOP Message from the Director Information Faculty list Research Projects International Conference Entrance Exam Visitors Publication Job Vacancy International Partnerships Links Access HANDBOOK FOR INTERNATIONAL RESEARCHERS Map of Inuyama
TOPICS
BONOBO Chimpanzee "Ai" Crania photos Itani Jun'ichiro archives Guidelines for Care and Use of Nonhuman Primates(pdf) Study material catalogue/database Guideline for field research of non-human primates Primate Genome DB

Primate Research Institute, Kyoto University
Inuyama, Aichi 484-8506, JAPAN
TEL. +81-568-63-0567
(Administrative Office)
FAX. +81-568-63-0085

Copyright (c)
Primate Research Institute,
Kyoto University All rights reserved.


Contact

Japanese

Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria

Thapana W, Sujiwattanarat P, Srikulnath K, Hirai H and *Koga A

For accurate analyses of eukaryotic tandem-repeat DNA, it is often required to clone a genomic DNA fragment into a bacterial plasmid. It is, however, a serious problem that tandem-repeat DNA is frequently subjected to structural changes during maintenance or amplification in the host bacteria. Here, we show an example of a clear difference in the instability of tandem-repeat DNA between different culturing temperatures. A fragment of monkey centromeric DNA carried by pUC19 was considerably degraded by culturing bacteria at 37oC, but the damage was reduced at 25oC. Thus, culturing temperature is a significant factor for avoiding degradation, in addition to the genotype of the host bacteria

Genetics Research 96: e13 (2014)

OCT/29/2014

Copyright(C) 2014 PRI (). All rights reserved.